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Enabling Methods

Our catalogue of laboratory methods provides access to some methods in immunology (e.g. CFSE proliferation assay, multiplex bead array, MDS assay), in molecular biology (e.g. DNA fingerprinting, PCR), in microscopy (e.g. confocal microscopy, two-photon microscopy), on centrifugation and PBMC collection.

Content Topics addressed Quiz Number of pages
Blood collection Venous sampling
Capillary sampling
No 8 pages
Other specimen collection Anoscopy
Ascites
Bone marrow
CSF
Cervical fluid
Fine needle aspiration
Semen
Urine
Stool
Pleural fluid
Throat and nasopharynx smears
Synovial fluid
Sigmoidoscopy
No 14 pages
PBMC separation Creating the gradient (Ficoll Hypaque method / Accuspin method)
Plasma & buffy coat removal
PBMC counting
No 5 pages
Centrifugation Principles of centrifugation
Centrifuges
Applications
Operation
No 20 pages
Imaging methodology Computed Tomography
Magnetic Resonance Imaging
Positron Emission Tomography
Confocal microscopy
Two photon microscopy
No 5 pages
Immunological methods CFSE proliferation assay
Multiplex bead array
MSD assay
Elispot assay
ELISA
Chromium release
Intracellular Cytokine Staining
T cell antigen preparations
Tetramer staining
Polyclonal expansion of CD8 T cells
No 10 pages
Molecular biology methods DNA fingerpriting
PCR
FISH
Nucleic acid sequence-based amplification
RNase protection assay
ChIP
Electrophoretic mobility shift assay
No 7 pages
Binding measurement methods Experimental principle No 1 page
Urine analysis This chapter is from Urosurf No 70 pages + 15 clinical examples