Enabling methods
Our catalogue of laboratory methods provides access to some methods in immunology (e.g. CFSE proliferation assay, multiplex bead array, MDS assay), in molecular biology (e.g. DNA fingerprinting, PCR), in microscopy (e.g. confocal microscopy, two-photon microscopy), on centrifugation and PBMC collection.
| Content | Topics addressed | Quiz | Number of pages |
|---|---|---|---|
| Blood collection | Venous sampling Capillary sampling |
No | 8 pages |
| Other specimen collection | Anoscopy Ascites Bone marrow CSF Cervical fluid Fine needle aspiration Semen Urine Stool Pleural fluid Throat and nasopharynx smears Synovial fluid Sigmoidoscopy |
No | 14 pages |
| PBMC separation | Creating the gradient (Ficoll Hypaque method / Accuspin method) Plasma & buffy coat removal PBMC counting |
No | 5 pages |
| Centrifugation | Principles of centrifugation Centrifuges Applications Operation |
No | 20 pages |
| Imaging methodology | Computed Tomography Magnetic Resonance Imaging Positron Emission Tomography Confocal microscopy Two photon microscopy |
No | 5 pages |
| Immunological methods | CFSE proliferation assay Multiplex bead array MSD assay Elispot assay ELISA Chromium release Intracellular Cytokine Staining T cell antigen preparations Tetramer staining Polyclonal expansion of CD8 T cells |
No | 10 pages |
| Molecular biology methods | DNA fingerpriting PCR FISH Nucleic acid sequence-based amplification RNase protection assay ChIP Electrophoretic mobility shift assay |
No | 7 pages |
| Binding measurement methods | Experimental principle | No | 1 page |
| Urine analysis | This chapter is from Urosurf | No | 70 pages + 15 clinical examples |